First-Principles Approach to Graded-Particle Composites (Completed) We aim at developing new theories to account for the effects of gradation and dielectric anisotropy on the effective dielectric response of composites. In these composites, the material properties of the particles can vary continuously in space, and have attracted much interest as one of the advanced inhomogeneous composite materials in various engineering applications. We will focus on the electrokinetic behaviors of graded cells, as well as the nonlinear optical properties of composites of graded particles. These problems have wide practical applications and importance, since identification and analysis of cell populations and (micro)biological particles are essential in diverse applications ranging from cancer research to chemical analysis of environmental pollutants. In addition to biological and environmental applications, electrokinetic phenomena have been suggested as possible mechanisms for nanomotors. The composite of graded particles with dielectric anisotropy possesses a large optical nonlinearity and thus can be a good candidate for nonlinear optical switching devices in photonics and real-time coherent optical signal processors.